منابع مشابه
Mounting and storage of gastrocamera films.
The various models of the Olympus gastrocamera use film of either 4 or 5 mm size, with 32 exposures per roll of film. The developed film is returned from the processing laboratory, therefore, as a film strip, 4 or 5 mm in width, and approximately 34 cm in length. The Olympus Camera Company supply a viewer with a magnifying power of x 10 with each instrument, and this is of some value in examini...
متن کاملMacromolecular Cryocrystallography: Cooling, Mounting, Storage and Transportation of Crystals
Simple methods are presented for handling, mounting, storage and transportation of crystals at cryogenic temperatures. They are easy to learn and have a number of technical and operational advantages over currently popular methods. In particular, the temperature of the crystal throughout all manipulations is known; it is shown never to rise above that of the warmest component of the cryogenic s...
متن کاملOptimal clearing and mounting media for confocal microscopy of thick specimens
Sample preparation for confocal microscopy typically involves fixing and staining, followed by clearing and mounting. Clearing involves treatment with solvent to dissolve lipids and other structures that cause light scattering and reduce the ability to image through thick specimens. Mounting involves transfer of the specimen into a medium which matches the refractive index the objective is desi...
متن کاملFixation, mounting and sealing with nail polish of cell specimens lead to incorrect FRET measurements using acceptor photobleaching.
Fluorescence resonance energy transfer (FRET) is a technique used for the study of functional interactions between molecules. The intimate vicinity between two fluorescent molecules (FRET-pair; donor and acceptor) allows for an energy transfer, which can be directly calculated as the so called FRET efficiency. This technique is used in fixed as well as living cells. Here we show first, measured...
متن کاملPolystyrene mounting medium.
Polystyrene mounting medium obtainable Ltd, 20 Du The medium described by Kirkpatrick One pounc and Lendrum (1939) and named by them mountant DPX (Distrene plasticised in xylene) was (75°% one replaced by a better formula in 1941. This medium BPS (butyl phthalate plasticised styrene) has given excellent and economical service until recently. The current trouble was not due to the makers of the ...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 1996
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s155192950006329x